How to prevent contamination while pipetting?
To minimize the risk of sample-to-pipette contamination, the following precautions should be followed:
- Always release the pipette’s push button slowly to prevent aerosol formation and uncontrolled liquid splashing within the pipette tip.
- Hold the pipette in a vertical position during pipetting and store the pipette in an upright position. This prevents liquids from running into the pipette body.
- Use filter tips or positive displacement tips to prevent the aerosol transfer from the sample into the pipette body.