Pipettes Sterilized (Autoclaving) is a sterilization technique in which equipment is sterilized at 121° C for 20 minutes in a high-pressure steam sterilizer (Autoclave Machine) where bacteria are killed by steam at elevated temperature and pressure. This is the most efficient sterilizing approach if you wish to eradicate the pipet or dispenser tip infection.
By heating and sterilizing at 100 °C, it is also helpful in eliminating germs, spores, or pathogens that are difficult to eradicate using standard decontamination procedures. When different microbes (bacteria, fungi, viruses, and so on) infect a microbe sample, the results can be influenced. Pipette and its tips, various research apparatus, and culture medium to be used in tests should be autoclave sterilized to avoid infection and cross-contamination.